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Protein A binds specifically to Fc regions of many mammalian immunoglobulins. Protein A- conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. Protein A-Separopore 4B-CL is used for purification and/or fractionation of IgG subclasses. It is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. This is also commonly used to capture immune complexes generated in immunoprecipitation (IP) experiments.
• Ligand: Native protein A (from Staphylococcus aureus, 42 kDa)
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Cyanogen bromide
• Molecular weight range: 6 x 104 - 2 x 107
• Ligand density: 3 mg Protein A / ml drained gel
• Binding capacity: 20 mg human IgG / ml drained gel
• pH stability: 3 - 9 (for use), 2 - 10 (for cleaning)
• Storage: 2 - 8 °C
• Supplied as lyophilized powder
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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