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Papain-Separopore 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. The advantages of using this Papain-Separopore 4B matrix is that it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the papain or controlling the flow of sample over an immobilized papain column. Papain-Separopore is also more stable against heat-induced denaturation, resulting in longer maintenance of activity.
• Ligand: Papain 21 kDa protein from Papaya latex (Carica papaya)
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 µm
• One unit of Papain will hydrolyze 1 mole of BAEE / min at pH 6.2 at 25 °C
• Ligand density: 3,000 - 5,000 units per ml Separopore (at pH 7 at 30 °C)
• Inhibitors of Papain: Heavy metals, carbonyls, NEM, p-chloromercuro-benzoate, E-64
• pH optimum 6 - 7
• Storage temp: 2 - 8 °C
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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