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Ni2+-chelated Separopore 4B-CL is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to nickel metal ions. Ni2+-chelated Separopore 4B-CL is ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Chelating group: Nickel
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Matrix activation: Epoxy
• Particle size range: 52 - 165 µm
• Metal ion capacity: >20µmol Ni2+ / ml drained gel
• Binding capacity: 5 - 8 mg (His)6-tagged protein / ml medium
• pH stability: 2 - 11
• Chemical stability: For one week at 40 °C: 0.01M HCl, 0.1M NaOH
For 12 h: 1M NaOH, 70% acetic acid.
For 30 min tested: 30% 2-propanol.
For 1 h tested: 2% SDS
• Compatibility during use: Stable in all commonly used buffers, reducing agents, denaturants, and detergents
• Supplied as suspension in 20% ethanol
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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