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bioWORLD<sup>®</sup> Ni-IMAC 2mL Columns

bioWORLD® Ni-IMAC 2mL Columns

Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.

Technical Specifications
• Imminodiacetate immobilized matrix charged with Ni metal ions.
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked Separopore 6B
• Particle size (range): 50-170µm
• Metal ion capacity: > 20µmol Ni2+/ml gel
• Spacer arm: 14 atom
• pH stability: 3-12
• Binding Capacity: Approx. 40 mg His-tagged protein/ml medium
• Supplied as suspension in 20% ethanol.
• Storage: 2 -8 °C

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
20170006-1 Ni-IMAC 5 x 2mL Columns, 5 Column - 1 ea

Specifications

20170006-1 $314.95 EA

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Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.

Technical Specifications
• Imminodiacetate immobilized matrix charged with Ni metal ions.
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked Separopore 6B
• Particle size (range): 50-170µm
• Metal ion capacity: > 20µmol Ni2+/ml gel
• Spacer arm: 14 atom
• pH stability: 3-12
• Binding Capacity: Approx. 40 mg His-tagged protein/ml medium
• Supplied as suspension in 20% ethanol.
• Storage: 2 -8 °C

Image(s) are representative of the product group and not necessarily the individual product.

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