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Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Imminodiacetate immobilized matrix charged with Ni metal ions.
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked Separopore 6B
• Particle size (range): 50-170µm
• Metal ion capacity: > 20µmol Ni2+/ml gel
• Spacer arm: 14 atom
• pH stability: 3-12
• Binding Capacity: Approx. 40 mg His-tagged protein/ml medium
• Supplied as suspension in 20% ethanol.
• Storage: 2 -8 °C
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
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