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MagneZoom™ Protein-A Kit is ideal for small-scale antibody purification and immunoprecipitation of proteins, protein complexes or other antigens. Paramagnetic beads of average 5 m in diameter, covalently coated with recombinant Protein A. Recombinant Protein A contains five high-affinity IgG binding domains to reduce nonspecific binding.
• High capacity - High binding capacity than typical magnetic beads from other suppliers
• Low non-specific binding - pre-blocked beads provide clean purification without non-specific binding (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins)
• Compatibility -bio-world's paramagnetic beads are compatible with manual and automated applications
• Consistency - magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods using centrifugation
• Storage: 2-8°C
• IP and Co-IP experiments
• Immunoprecipitation in non-reducing conditions
• Antibody purification
• MagneZoom™ -A (25mg/ml): 1ml
• Binding/Wash Buffer
• Elution Buffer
• Neutralization Buffer
• 1X SDS Sample Buffer
This kit requires MagneZoom™ paramagnetic bead station (bioWorld Mnf. No. 20160000-1) for separation of beads.
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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