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bioWORLD<sup>®</sup> L-Histidine Separopore<sup>®</sup> (Agarose) 4B-CL

bioWORLD® L-Histidine Separopore® (Agarose) 4B-CL

Histidine-Separopore 4B-CL can be used in the purification of supercoiled (sc) plasmid DNA (pDNA) from Escherichia coli lysates. This can also be used for the separation of IgG from human plasma and selective purification of bioactive oligouronides such as -(1, 4)-oligogalacturonides. It is used for the purification of histidine binding proteins. This matrix can also be used for the purification of proteins expressed in Escherichia coli that use the coordination of a heme tag. This approach provides an affinity purification tag visible to the eye, facilitating tracking of the protein.

Technical Specifications:
• Ligand: L-Histidine
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Matrix active group: Epoxy
• Matrix spacer: 12 atoms
• Attachment: Amino
• Extent of coupling: 1 - 2 mol / ml
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as a suspension in 2M NaCl, 0.02% thimerosal
• Storage: 2 - 8 °C

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
20181069-1 L-Histidine Separopore(R) (Agarose) 4B-CL, 5 mL - 1 ea

Specifications

20181069-1 $165.20 EA
20181069-2 L-Histidine Separopore(R) (Agarose) 4B-CL, 10 mL - 1 ea

Specifications

20181069-2 $226.05 EA
20181069-3 L-Histidine Separopore(R) (Agarose) 4B-CL, 25 mL - 1 ea

Specifications

20181069-3 $450.60 EA

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Histidine-Separopore 4B-CL can be used in the purification of supercoiled (sc) plasmid DNA (pDNA) from Escherichia coli lysates. This can also be used for the separation of IgG from human plasma and selective purification of bioactive oligouronides such as -(1, 4)-oligogalacturonides. It is used for the purification of histidine binding proteins. This matrix can also be used for the purification of proteins expressed in Escherichia coli that use the coordination of a heme tag. This approach provides an affinity purification tag visible to the eye, facilitating tracking of the protein.

Technical Specifications:
• Ligand: L-Histidine
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Matrix active group: Epoxy
• Matrix spacer: 12 atoms
• Attachment: Amino
• Extent of coupling: 1 - 2 mol / ml
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as a suspension in 2M NaCl, 0.02% thimerosal
• Storage: 2 - 8 °C

Image(s) are representative of the product group and not necessarily the individual product.

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