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Description Back to Top
bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains commonly used for protein expression. The coupling chemistry is leak-resistant and provides a matrix with very low nonspecific binding. A column of immobilized E. coli lysate thus functions as a reverse affinity matrix. The contaminating E. coli antibodies are absorbed onto the matrix and the purified antisera passes through for further use.
• Ligand: E. coli proteins that are extracted from three strains
• Matrix: Separopore 4B (agarose beads, 4 %)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Matrix activation: Cyanogen bromide
• pH stability: 4 - 9
• Storage temp: 4 °C in the presence of sodium azide
• Supplied as settled gel in PBS containing sodium azide
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Shipping Back to Top
Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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