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bioWORLD<sup>®</sup> E. coli protein Separopore<sup>®</sup> (Agarose)

bioWORLD® E. coli protein Separopore® (Agarose)

bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains commonly used for protein expression. The coupling chemistry is leak-resistant and provides a matrix with very low nonspecific binding. A column of immobilized E. coli lysate thus functions as a reverse affinity matrix. The contaminating E. coli antibodies are absorbed onto the matrix and the purified antisera passes through for further use.

Technical specifications:
• Ligand: E. coli proteins that are extracted from three strains
• Matrix: Separopore 4B (agarose beads, 4 %)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Matrix activation: Cyanogen bromide
• pH stability: 4 - 9
• Storage temp: 4 °C in the presence of sodium azide
• Supplied as settled gel in PBS containing sodium azide

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
20181006-1 E. coli Protein Separopore(R) (Agarose) 4B, 1 mL - 1 ea

Specifications

20181006-1 $154.70 EA
20181006-2 E. coli Protein Separopore(R) (Agarose) 4B, 5 mL - 1 ea

Specifications

20181006-2 $301.45 EA
20181006-3 E. coli Protein Separopore(R) (Agarose) 4B, 10 mL - 1 ea

Specifications

20181006-3 $489.35 EA

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bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains commonly used for protein expression. The coupling chemistry is leak-resistant and provides a matrix with very low nonspecific binding. A column of immobilized E. coli lysate thus functions as a reverse affinity matrix. The contaminating E. coli antibodies are absorbed onto the matrix and the purified antisera passes through for further use.

Technical specifications:
• Ligand: E. coli proteins that are extracted from three strains
• Matrix: Separopore 4B (agarose beads, 4 %)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 104 - 2 x 107
• Matrix activation: Cyanogen bromide
• pH stability: 4 - 9
• Storage temp: 4 °C in the presence of sodium azide
• Supplied as settled gel in PBS containing sodium azide

Image(s) are representative of the product group and not necessarily the individual product.

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