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Cu2+-chelated Separopore 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to Cu2+ metal ions. It is suitable for both laboratory scale purification and large-scale use.
• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Cu2+ metal ions.
• Ideal for purification of recombinant proteins containing (His)6-fusion tag.
• It provides effective binding and selectivity for a wide range of proteins.
• High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).
• Ligand: Copper
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy
• Spacer arm: 14 atoms
• Ligand density: > 20 µmol Cu2+ / ml drained gel
• Binding capacity: 5 mg histidine-tagged protein / ml drained gel
• pH stability: 3 - 12
• Chemical Stability: 40 °C for 7 days in 0.01M HCl, 1M NaOH, and acetate buffer pH 4, 20% ethanol
• Autoclavable at 121 °C in 20% ethanol
• Antimicrobial agent: 20% ethanol
• Supplied as suspension in 20% ethanol
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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