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bioWORLD<sup>®</sup> Co2+-Chelated Separopore<sup>®</sup> (Agarose) 4B-CL

bioWORLD® Co2+-Chelated Separopore® (Agarose) 4B-CL

Co2+-chelated Separopore 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to metal ions. It is suitable for both laboratory scale purification and large-scale use.

Properties:
• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Co2+ metal ions.
• Ideal for purification of recombinant proteins containing (His)6-fusion tag.
• It provides effective binding and selectivity for a wide range of proteins.
• High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).

Technical specifications:
• Ligand: Cobalt
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy
• Spacer arm: 14 atoms
• Ligand density: > 20 µmol Co2+ / ml drained gel
• Binding capacity: 5 mg histidine-tagged protein / ml drained gel
• pH stability: 3 - 12
• Chemical Stability: 40 °C for 7 days in 0.01M HCl, 1M NaOH, and acetate buffer pH 4, 20% ethanol
• Autoclavable at 121 °C in 20% ethanol
• Antimicrobial agent: 20% ethanol
• Supplied as suspension in 20% ethanol

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
20181047-1 Co2+-Chelated Separopore(R) (Agarose) 4B-CL, 10 mL - 1 ea

Specifications

20181047-1 $187.95 EA
20181047-2 Co2+-Chelated Separopore(R) (Agarose) 4B-CL, 25 mL - 1 ea

Specifications

20181047-2 $294.15 EA
20181047-3 Co2+-Chelated Separopore(R) (Agarose) 4B-CL, 50 mL - 1 ea

Specifications

20181047-3 $483.45 EA

Description Back to Top

Co2+-chelated Separopore 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that separates proteins via their differences in affinity to metal ions. It is suitable for both laboratory scale purification and large-scale use.

Properties:
• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Co2+ metal ions.
• Ideal for purification of recombinant proteins containing (His)6-fusion tag.
• It provides effective binding and selectivity for a wide range of proteins.
• High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).

Technical specifications:
• Ligand: Cobalt
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy
• Spacer arm: 14 atoms
• Ligand density: > 20 µmol Co2+ / ml drained gel
• Binding capacity: 5 mg histidine-tagged protein / ml drained gel
• pH stability: 3 - 12
• Chemical Stability: 40 °C for 7 days in 0.01M HCl, 1M NaOH, and acetate buffer pH 4, 20% ethanol
• Autoclavable at 121 °C in 20% ethanol
• Antimicrobial agent: 20% ethanol
• Supplied as suspension in 20% ethanol

Image(s) are representative of the product group and not necessarily the individual product.

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