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Pre-activated medium for immobilization of ligands containing primary amines. N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation. No charge is introduced into the matrix after the coupling reaction. No ligand leakage after coupling. Superior stability: Activated matrix is much more resistant to hydrolysis. Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids. Coupling reaction can also be carried out in organic solvents. High coupling efficiency and easy to handle. CDI-activated Separopore 4B is activated with CDI groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles.
• Group to be coupled: -NH2 groups
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 m
• Molecular weight range: 6 x 104 - 2 x 107
• Coupling conditions: pH: 8.5 - 11, Temp: 4 - 25 °C, Time: 6 - 24 h
• pH stability: 11 (ligand dependent)
• Storage: 2 - 8 °C
• Supplied as suspension in acetone
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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