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bioWORLD<sup>®</sup> Brown Separopore<sup>®</sup> (Agarose) 4B-CL

bioWORLD® Brown Separopore® (Agarose) 4B-CL

Brown-Separopore 4B-CL is a dye-ligand affinity matrix consisting of Reactive Brown 10 bound to cross-linked 4% agarose beads. It is used to purify proteins that have distinctive affinity to this dye. Immobilized dyes have been found to bind from 5 to 60 % of the proteins in various crude cell extracts. The affinity for reactive dyes to proteins may be due to substrate /cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins.

Technical specifications:
• Ligand: Reactive Brown 10
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Ligand density: 4 - 5 mg Reactive Brown 10 / ml drained medium
• Binding capacity: 3 - 5 mg human serum albumin/ ml gel
• pH stability: 3 - 13
• Storage: 2 - 8 °C
• Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
20181100-1 Brown Separopore(R) (Agarose) 4B-CL, 5 mL - 1 ea

Specifications

20181100-1 $64.30 EA
20181100-2 Brown Separopore(R) (Agarose) 4B-CL, 25 mL - 1 ea

Specifications

20181100-2 $163.55 EA

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Brown-Separopore 4B-CL is a dye-ligand affinity matrix consisting of Reactive Brown 10 bound to cross-linked 4% agarose beads. It is used to purify proteins that have distinctive affinity to this dye. Immobilized dyes have been found to bind from 5 to 60 % of the proteins in various crude cell extracts. The affinity for reactive dyes to proteins may be due to substrate /cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins.

Technical specifications:
• Ligand: Reactive Brown 10
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Ligand density: 4 - 5 mg Reactive Brown 10 / ml drained medium
• Binding capacity: 3 - 5 mg human serum albumin/ ml gel
• pH stability: 3 - 13
• Storage: 2 - 8 °C
• Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal

Image(s) are representative of the product group and not necessarily the individual product.

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