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Description Back to Top
Brown-Separopore 4B-CL is a dye-ligand affinity matrix consisting of Reactive Brown 10 bound to cross-linked 4% agarose beads. It is used to purify proteins that have distinctive affinity to this dye. Immobilized dyes have been found to bind from 5 to 60 % of the proteins in various crude cell extracts. The affinity for reactive dyes to proteins may be due to substrate /cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins.
• Ligand: Reactive Brown 10
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Ligand density: 4 - 5 mg Reactive Brown 10 / ml drained medium
• Binding capacity: 3 - 5 mg human serum albumin/ ml gel
• pH stability: 3 - 13
• Storage: 2 - 8 °C
• Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
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Shipping Back to Top
Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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