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Description Back to Top
Blue-Separopore 4B-CL is Cibacron™ Blue F3G-A covalently attached to the Separopore 4B-CL matrix by the triazine coupling method. The structure of the blue dye makes it a very versatile tool for separating many proteins, e.g. albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+. The highly cross-linked matrix provides a stable, rigid medium.
Blue-Separopore 4B-CL is a dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins. The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore 4B-CL via chlorotriazine ring. Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool often used for the removal of BSA from serum for the enrichment of less abundant proteins.
• Ligand: Cibacron Blue F3G-A
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Triazine coupling
• Ligand density: 2 - 6 µmol Cibacron blue F3G-A / ml drained gel
• Binding capacity: 15 mg bovine serum albumin / ml drained gel
• pH stability: 3 - 13
• Storage: 2 - 8 °C
• Supplied as 50% slurry in 0.5M NaCl containing 0.02% thimerosal
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Shipping Back to Top
Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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