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Magnetic beads are prepared by covalent immobilization of native protein G (from Streptococcus sp.) to BlackPearl Magnetic Beads by CNBr activation coupling chemistry.
Protein G shows strong binding affinity to Fc region of several classes of mammalian immunoglobulins, BlackPearl has been used as a reliable tool to purify monoclonal and polyclonal IgGs from ascites, serum and cell culture supernatants. It is an ideal choice for affinity purification and/or fractionation of IgG subclasses which binds poorly to protein A such as bovine, goat, rat, and sheep IgG.
These products are supplied as suspension in a buffer containing 100 mM Tris Cl, pH 8.0, 500 mM NaCl, 0.05 % NaN3, and 0.02 % thimerosal.
• Ligand: Native protein G from Streptococcus sp.
• Ligand coupling method: CNBr activation
• Binding capacity: Approximately 20 mg human IgG/ml drained gel
• Matrix: Magnetic agarose beads, 6%
• Particle size (range): 50-125 µm
• Suspension: 25% slurry (1ml contains 250uL settled vol.)
• pH stability: 3-9 (for use), 2-10 (for cleaning)
• Storage: 2-8 °C
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Image(s) are representative of the product group and not necessarily the individual product.
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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