We manufacture and distribute fine chemicals and laboratory
products - with quality and delivery you can count on every time.

Corner
bioWORLD<sup>®</sup> Benzamidine Separopore<sup>®</sup> 6B(columns)

bioWORLD® Benzamidine Separopore® 6B(columns)

Benzamidine-Separopore 6B is p-aminobenzamidine covalently attached to Separopore 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine-Separopore 6B and can thus be used for purification and/or removal of these substances.
Benzamidine-Separopore 6B can be used for purifying serine proteases such as trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain. For recombinant purification, Benzamidine-Separopore 6B columns can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags.

Benzamiding-Separopore 6B is tested for its binding capacity for trypsin, but the resin has been used in the purification of a variety of proteins including thrombin, Adenylyl cyclase-activating protease from bovine sperm, Fibrinolytic enzyme from Agkistrodon contortrix, Tryptase from rat skin, Serine endopeptidase from rat mammary tissue, Glycosylation-enhancing factor (GEF), Plasminogen activator from embryo lung culture, Plasminogen activator from human umbilical vein endothelial cells and Thrombin-like enzyme from Bothrops atrox venom

Technical specifications:
• Ligand: p-Aminobenzamidine (PAB)
• Matrix: Separopore 6B (agarose beads, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy activation
• Ligand density: 7 mole p-aminobenzamidine/ml drained gel
• Binding capacity: > 10mg trypsin/ml drained gel
• Exclusion limit: approximately 2 x 107
• pH stability: Long term: 3 - 11; Short term: 2 - 13
• Chemical stability: Stable to all commonly used aqueous buffers
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Storage temp: 4 -8 °C
• Benzamidine-Separopore 6B is supplied 2 ml column in 20% ethanol

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
20170024-1 Benzamidine Separopore (R) 6B (Columns), 2 mL - 1 ea

Specifications

20170024-1 $145.20 EA
20170024-2 Benzamidine Separopore (R) 6B (Columns), 5 mL - 1 ea

Specifications

20170024-2 $195.25 EA

Description Back to Top

Benzamidine-Separopore 6B is p-aminobenzamidine covalently attached to Separopore 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine-Separopore 6B and can thus be used for purification and/or removal of these substances.
Benzamidine-Separopore 6B can be used for purifying serine proteases such as trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain. For recombinant purification, Benzamidine-Separopore 6B columns can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags.

Benzamiding-Separopore 6B is tested for its binding capacity for trypsin, but the resin has been used in the purification of a variety of proteins including thrombin, Adenylyl cyclase-activating protease from bovine sperm, Fibrinolytic enzyme from Agkistrodon contortrix, Tryptase from rat skin, Serine endopeptidase from rat mammary tissue, Glycosylation-enhancing factor (GEF), Plasminogen activator from embryo lung culture, Plasminogen activator from human umbilical vein endothelial cells and Thrombin-like enzyme from Bothrops atrox venom

Technical specifications:
• Ligand: p-Aminobenzamidine (PAB)
• Matrix: Separopore 6B (agarose beads, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy activation
• Ligand density: 7 mole p-aminobenzamidine/ml drained gel
• Binding capacity: > 10mg trypsin/ml drained gel
• Exclusion limit: approximately 2 x 107
• pH stability: Long term: 3 - 11; Short term: 2 - 13
• Chemical stability: Stable to all commonly used aqueous buffers
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Storage temp: 4 -8 °C
• Benzamidine-Separopore 6B is supplied 2 ml column in 20% ethanol

Image(s) are representative of the product group and not necessarily the individual product.

Shipping Back to Top

Shipping Costs
Click here for Shipping Costs
314029 has a rating of 4.9 stars based on 10 reviews.