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Benzamidine-Separopore 6B is p-aminobenzamidine covalently attached to Separopore 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine-Separopore 6B and can thus be used for purification and/or removal of these substances.
Benzamidine-Separopore 6B can be used for purifying serine proteases such as trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain. For recombinant purification, Benzamidine-Separopore 6B columns can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags.
Benzamiding-Separopore 6B is tested for its binding capacity for trypsin, but the resin has been used in the purification of a variety of proteins including thrombin, Adenylyl cyclase-activating protease from bovine sperm, Fibrinolytic enzyme from Agkistrodon contortrix, Tryptase from rat skin, Serine endopeptidase from rat mammary tissue, Glycosylation-enhancing factor (GEF), Plasminogen activator from embryo lung culture, Plasminogen activator from human umbilical vein endothelial cells and Thrombin-like enzyme from Bothrops atrox venom
• Ligand: p-Aminobenzamidine (PAB)
• Matrix: Separopore 6B (agarose beads, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy activation
• Ligand density: 7 mole p-aminobenzamidine/ml drained gel
• Binding capacity: > 10mg trypsin/ml drained gel
• Exclusion limit: approximately 2 x 107
• pH stability: Long term: 3 - 11; Short term: 2 - 13
• Chemical stability: Stable to all commonly used aqueous buffers
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Storage temp: 4 -8 °C
• Benzamidine-Separopore 6B is supplied 2 ml column in 20% ethanol
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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