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bioWORLD<sup>®</sup> AMP-Separopore<sup>®</sup> (Agarose) 4B-CL (Lyophilized)

bioWORLD® AMP-Separopore® (Agarose) 4B-CL (Lyophilized)

AMP Separopore interacts strongly with NAD+-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD+ or NADP+ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5'-AMP Separopore. The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-(6-aminohexyl)carbamoylmethyl).

Technical Specifications:
• Ligand: Adenosine Monophosphate (AMP)
• Matrix: Separopore 4B-CL (highly crosslinked agarose beads, 4%).
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy
• Matrix attachment: N6
• Spacer arm: 1, 6-diaminohexane
• Ligand density: 2 µmol AMP / ml drained gel
• Binding capacity: 5-7 mg lactate dehydrogenase / ml drained gel
• pH stability: 4 - 10
• Flow rate specifications: 70 - 140 cm / h.
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Supplied as lyophilized powder and 1g yields 8-10 ml of gel

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
20181079-1 AMP Separopore(R) (Agarose) 4B-CL (Lyophilized), 1 g - 1 ea

Specifications

20181079-1 $347.75 EA
20181079-2 AMP Separopore(R) (Agarose) 4B-CL (Lyophilized), 3 g - 1 ea

Specifications

20181079-2 $610.45 EA

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AMP Separopore interacts strongly with NAD+-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD+ or NADP+ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5'-AMP Separopore. The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-(6-aminohexyl)carbamoylmethyl).

Technical Specifications:
• Ligand: Adenosine Monophosphate (AMP)
• Matrix: Separopore 4B-CL (highly crosslinked agarose beads, 4%).
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy
• Matrix attachment: N6
• Spacer arm: 1, 6-diaminohexane
• Ligand density: 2 µmol AMP / ml drained gel
• Binding capacity: 5-7 mg lactate dehydrogenase / ml drained gel
• pH stability: 4 - 10
• Flow rate specifications: 70 - 140 cm / h.
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Supplied as lyophilized powder and 1g yields 8-10 ml of gel

Image(s) are representative of the product group and not necessarily the individual product.

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