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2-Iminobiotin-Separopore 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore than the biotin resin. It is also used for removal of free avidin from (strept)avidin conjugate preparations. Interaction with avidin and streptavidin is pH dependent: forms stable complex above pH 9.5 and dissociates at pH 4.
• Ligand: 2-Iminobiotin
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Amino
• Molecular weight range: 6 x 104 - 2 x 107
• Binding capacity: 4 mg avidin / ml drained gel
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as suspension in 0.5M NaCl, 0.01M sodium phosphate (pH 6.8) and 0.02% sodium azide
• Appearance: White to off-white gel suspension
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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