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• Intended Use: Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella
spp. Conforms to Harmonized USP/EP/JP Requirements.
• Product Summary and Explanation: Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by
Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis
modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later
showed that incubation at 41.5 0.5°C for 24 hours improved recovery of Salmonella spp.
Rappaport-Vassiliadis Salmonella Enrichment Broth is a modification of Rappaport-Vassiliadis R10 Broth, where Soy Peptone has replaced Enzymatic Digest of Casein as the nitrogen and vitamin source. Soy Peptone has shown to enhance the growth of Salmonella spp. and minimizes Bovine Spongiform Encephalopathy (BSE) risk. This medium selectively enriches for Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green, high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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