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• Intended Use: PPLO Broth W/O CV is used with enrichments for the isolation and cultivation of Mycoplasma spp.
• Product Summary and Explanation: PPLO Broth W/O CV is prepared according to the formula described by Morton and Lecce.
Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as pleuropneumonia-like organism or PPLO. Mycoplasmas belong to the class of Mollicutes soft skin, which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 micromillimeters.
Mycoplasma pneumoniae is a common cause of mild pneumonia and usually affects people younger than 40.5 Studies suggest that it causes 15 - 50% of all pneumonia in adults and an even higher percentage of pneumoniae in school-aged children. The symptoms include headache, fever, cough, chest pain, and sore throat.
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Spectrum Chemical Mfg. Corp. offers a flat fee shipping when items are shipped “directly from Spectrum warehouses” by regular UPS ground within the contiguous United States. There are some additional charges that are item specific. Not included are any expedited or LTL shipments or dIrect shipped items from Spectrum vendors or manufacturers which are charged at published carrier rates.
|Order Sub-Total||Shipping Charge|
|$0 - <$299.99||$19.25|
|$300 - <$499.99||$32.50|
|$500 - <$999.99||$44.75|
|Additional Charge Items||Shipping Charge|
|Hazard Charge - Ground||$33.00 per box|
|Hazard Charge - Air||$46.50 per box|
|Poison Pack Charge (Small)||$15.00 per box|
|Poison Pack Charge (Large)||$25.00 per box|
|Ice Pack Charge (Small)||$10.00 per box|
|Ice Pack Charge (Large)||$15.00 per box|
|Pallet Charge (Wood Pallet)||$25.00 per box|
|Pallet Charge (Plastic Pallet)||$40.00 per box|
|Fuel Surcharge (<$400 subtotal)||$5.85|
|Fuel Surcharge (>$400 subtotal)||$10.55|
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