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acumedia<sup>®</sup> DNase Test Agar

acumedia® DNase Test Agar

• Intended Use: DNase Test Agar is used for the differentiation of microorganisms on the basis of deoxyribonuclease activity.
• Product Summary: In 1956, Weckman and Catlin showed a correlation between increased DNase activity of Staphylococcus
aureus and positive coagulase activity. Their research suggested DNase activity could be used to identify potentially pathogenic
staphylococci. DiSalvo confirmed their results by obtaining excellent correlation between coagulase and DNase activity of
staphylococci isolated from clinical specimens. Jeffries, Holtman, and Guse incorporated DNA in agar to study DNase production
by bacteria and fungi. Polymerized DNA precipitates in the presence of 1N HCl, creating an opaque medium. Organisms that
degrade DNA produce a clear zone around an inoculum streak. Fusillo and Weiss studied calcium requirements of staphylococci
for DNase production, and concluded additional calcium was unnecessary when a complete nutritive medium was used.

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
741-60242 DNase Test Agar, 500 g - 500 g

NCM0161A $122.30 EA
741-60242-5KG DNase Test Agar, 5 kg - 5 kg

NCM0161B $1109.40 EA
741-60242 DNase Test Agar, 10 kg - 10 kg

NCM0161C $2240.40 EA

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• Intended Use: DNase Test Agar is used for the differentiation of microorganisms on the basis of deoxyribonuclease activity.
• Product Summary: In 1956, Weckman and Catlin showed a correlation between increased DNase activity of Staphylococcus
aureus and positive coagulase activity. Their research suggested DNase activity could be used to identify potentially pathogenic
staphylococci. DiSalvo confirmed their results by obtaining excellent correlation between coagulase and DNase activity of
staphylococci isolated from clinical specimens. Jeffries, Holtman, and Guse incorporated DNA in agar to study DNase production
by bacteria and fungi. Polymerized DNA precipitates in the presence of 1N HCl, creating an opaque medium. Organisms that
degrade DNA produce a clear zone around an inoculum streak. Fusillo and Weiss studied calcium requirements of staphylococci
for DNase production, and concluded additional calcium was unnecessary when a complete nutritive medium was used.

Image(s) are representative of the product group and not necessarily the individual product.

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