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Victory Scientific PCR Master Mixes

Victory Scientific PCR Master Mixes

Adding to the innovative hardware technology for PCR instrumentation, reliable reagent technology is another key component to perform ultra-fast amplification and detection of genes in PCR applications. Genesystem offers a series of PCR master mixes for ultra-fast PCR tasks using GENECHECKER. Depending on the application, three different formats are available for immediate and easy use for your PCR applications: Rapi:Amp for General Thermal Cycling, Rapi:Detect for Real Time PCR, and Rapi:1-Step for One-Step RT PCR.

The Series of master mixes for Genesystem are a 2x concentrated, ready-to-use reaction cocktail containing all components, except primers and template, for ultra-fast PCR tasks. These premixes includes a novel antibody-mediated hot-start DNA polymerases with improved speed when compared to other commercial polymerases. The master mix for real-time detection includes a double strand DNA specific-binding fluorescent dye and also there is areverse transcriptase available for one-step RT-PCR applications. In addition, the Uracil-DNA glycosylase with dUTP system is applied to these PCR premixes for the prevention of carryover contamination. All the master mixes of Genesystem have been optimized to deliver maximum PCR efficiency, sensitivity and robust fluorescent signal using fast cycling protocols for dye-based detection in ultra-fast real-time PCR using GENECHECKER.

General Protocol:
• DNA Template: Your volume
• 2x Master Mix: 5 µL
• Forward Primer (5-10 pmoles/µL): 1 µL
• Reverse Primer (5-10 pmoles/µL): 1 µL
• D.W.: Adjust to Final 10 µL

Thermal Cycling and Real-Time PCR:
• cDNA Synthesis: 0 cycles (Set at 0 sec)
• Enzyme Activation: 1 cycle (95°C for 30 secs)
• PCR Amplification (Denaturation): 30 to 50 cycles (95°C for 3 to 10 secs)
• PCR Amplification (Annealing): 30 to 50 cycles (your temp for 3 to 10 secs)
• PCR Amplification (Extension): 30 to 50 cycles (72°C for 3 to 10 secs)

One Step RT-PCR:
• cDNA Synthesis: 1 cycle (50°C for 5 to 30 mins)
• Enzyme Activation: 1 cycle (95°C for 30 secs)
• PCR Amplification (Denaturation): 30 to 50 cycles (95°C for 3 to 10 secs)
• PCR Amplification (Annealing): 30 to 50 cycles (3 to 10 secs)
• PCR Amplification (Extension): 30 to 50 cycles (72°C for 3 to 10 secs)

Image(s) are representative of the product group and not necessarily the individual product.
Catalog Item Availability Mfr. Part # List Price Unit
581-10068-EA Victory Scientific Rapi:Amp Master Mix for UF-100 GENECHECKER (400 Reactions), 1 mL x 2 Tubes - 1 pk

004003 $378.35 EA
581-10062-EA Victory Scientific Rapi:Detect Master Mix with Fluorescent Dye for Model UF-150 (400 Reactions), 1 mL x 2 Tubes - 1 pk

004001 $418.50 EA
581-10065-EA Victory Scientific Rapi:1-Step One-step RT-PCR Mix with Fluorescent Dye for Model UF-150 (400 Reactions), 1 mL x 2 Tubes - 1 pk

004002 $635.55 EA
581-10071-EA Victory Scientific Rapi:Amp Master Mix with Gel loading Dye for UF-100 (400 Reactions) - 1 pk

004004 $418.50 EA
581-10074-EA Victory Scientific Rapi:Spec Probe Master Mix for Model UF-150 (400 Reactions) - 1 pk

004005 $418.50 EA
581-10077-EA Victory Scientific Rapi:1-Step Master Mix with RTase for Model UF-100 (400 Reactions) - 1 pk

004006 $533.10 EA
581-10080-EA Victory Scientific Rapi:Spec Probe Master Mix with RTase for UF-150 (400 Reactions) - 1 pk

004007 $635.55 EA
371-11396-PK Victory Scientific Rapi:Lyo Probe, Lyphillized Master Mix, For Use with UF-150 GENECHECKER (480 Reactions) - 48 ea

004105 $632.75 PK

Description Back to Top

Adding to the innovative hardware technology for PCR instrumentation, reliable reagent technology is another key component to perform ultra-fast amplification and detection of genes in PCR applications. Genesystem offers a series of PCR master mixes for ultra-fast PCR tasks using GENECHECKER. Depending on the application, three different formats are available for immediate and easy use for your PCR applications: Rapi:Amp for General Thermal Cycling, Rapi:Detect for Real Time PCR, and Rapi:1-Step for One-Step RT PCR.

The Series of master mixes for Genesystem are a 2x concentrated, ready-to-use reaction cocktail containing all components, except primers and template, for ultra-fast PCR tasks. These premixes includes a novel antibody-mediated hot-start DNA polymerases with improved speed when compared to other commercial polymerases. The master mix for real-time detection includes a double strand DNA specific-binding fluorescent dye and also there is areverse transcriptase available for one-step RT-PCR applications. In addition, the Uracil-DNA glycosylase with dUTP system is applied to these PCR premixes for the prevention of carryover contamination. All the master mixes of Genesystem have been optimized to deliver maximum PCR efficiency, sensitivity and robust fluorescent signal using fast cycling protocols for dye-based detection in ultra-fast real-time PCR using GENECHECKER.

General Protocol:
• DNA Template: Your volume
• 2x Master Mix: 5 µL
• Forward Primer (5-10 pmoles/µL): 1 µL
• Reverse Primer (5-10 pmoles/µL): 1 µL
• D.W.: Adjust to Final 10 µL

Thermal Cycling and Real-Time PCR:
• cDNA Synthesis: 0 cycles (Set at 0 sec)
• Enzyme Activation: 1 cycle (95°C for 30 secs)
• PCR Amplification (Denaturation): 30 to 50 cycles (95°C for 3 to 10 secs)
• PCR Amplification (Annealing): 30 to 50 cycles (your temp for 3 to 10 secs)
• PCR Amplification (Extension): 30 to 50 cycles (72°C for 3 to 10 secs)

One Step RT-PCR:
• cDNA Synthesis: 1 cycle (50°C for 5 to 30 mins)
• Enzyme Activation: 1 cycle (95°C for 30 secs)
• PCR Amplification (Denaturation): 30 to 50 cycles (95°C for 3 to 10 secs)
• PCR Amplification (Annealing): 30 to 50 cycles (3 to 10 secs)
• PCR Amplification (Extension): 30 to 50 cycles (72°C for 3 to 10 secs)

Image(s) are representative of the product group and not necessarily the individual product.

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